This document provides protocols supporting the use of external RNA controls in microarray and QRT-PCR-based gene expression experiments, including preparation of control transcripts, design of primers and amplicons, quality control, use in final experimental or clinical test application, and analysis and interpretation of data obtained. A CLSI-IFCC joint project.
CLSI MM16-A
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Use of External RNA Controls in Gene Expression Assays; Approved Guideline, MM16AE
Clinical and Laboratory Standards Institute , 01/01/2005